Exosomes Released from Mycoplasma Infected Tumor Cells Activate Inhibitory B Cells

Mycoplasmas cause numerous human diseases and are common opportunistic pathogens in cancer patients and immunocompromised individuals. Mycoplasma infection elicits various host immune responses. Here we demonstrate that mycoplasma-infected tumor cells release exosomes (myco+ exosomes) that specifically activate splenic B cells and induce splenocytes cytokine production. Induction of cytokines, including the proinflammatory IFN-γ and the anti-inflammatory IL-10, was largely dependent on the presence of B cells. B cells were the major IL-10 producers. In splenocytes from B cell deficient μMT mice, induction of IFN-γ+ T cells by myco+ exosomes was greatly increased compared with wild type splenocytes. In addition, anti-CD3-stimulated T cell proliferation was greatly inhibited in the presence of myco+ exosome-treated B cells. Also, anti-CD3-stimulated T cell signaling was impaired by myco+ exosome treatment. Proteomic analysis identified mycoplasma proteins in exosomes that potentially contribute to the effects. Our results demonstrate that mycoplasma-infected tumor cells release exosomes carrying mycoplasma components that preferentially activate B cells, which in turn, are able to inhibit T cell activity. These results suggest that mycoplasmas infecting tumor cells can exploit the exosome pathway to disseminate their own components and modulate the activity of immune cells, in particular, activate B cells with inhibitory activity

PHYSIOLOGICAL AND BIOCHEMICAL ASPECTS OF REPRODUCING THE WILD LONG-RHIZOME MORPHOTYPE OF <i> MEDICAGO FALCATA </i> L. UNDER CULTIVATION

Morphology, nitrogen-fixing activity and seed productivity of the introduced wild long-rhizome yellow alfalfa (Medicago falcata L.) under exogenous treatment with microbial preparations and phytohormone (homobrassinolide) were studied in field experiments. It was revealed that growth activators increase seed productivity and nitrogen-fixing activity in the cultivated taproot alfalfa type and, on the whole, have no effect on metabolism in the long-rhizome morphotype plants. To solve the problem it is necessary to develop Rhizobium preparations increasing nitrogen-fixing activity of the long-rhizome alfalfa plants on the basis of natural isolates and the use of phytohormones with due regard to the peculiarities of the ontogenesis of the long-rhizome M. falcata morphotype